|
| Status |
Public on Apr 29, 2012 |
| Title |
Trim28CKO unstimulated CD4 positve T cell rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Trim28 conditional KO mice naïve CD4 positive cell, unstimulated, replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Female
age: 6 weeks old
cell type: CD4 positive T cell
treatment: unstimulated
genotype: Trim28CKO
|
| Treatment protocol |
Stimulations were done with plate-coated anti-CD3(1mg/ml) and anti-CD28 (1mg/ml)for 13 hours.
|
| Growth protocol |
Mice were maintained under specific pathogen-free conditions according to institutional guidelines under approved protocols in Kyoto University.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted with Qiagen RNeasy mini kit with on-column DNaseI treatment. RNA quality was assessed using Agilent 2100 Bioanalyzer according to manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA was produced using Agilent Low Input QuickAmp Labeling Kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slide were washed and then scanned with an Agilent G2505B scanner
|
| Data processing |
Data were obtained and processed with the Agilent Feature Extraction Software(v9.5). The resulting data were imported into Expressionist and quantile normalized intensities were exported.
|
| |
|
| Submission date |
Sep 19, 2011 |
| Last update date |
Apr 29, 2012 |
| Contact name |
Tasuku Honjo |
| E-mail(s) |
honjo@mfour.med.kyoto-u.ac.jp
|
| Organization name |
Kyoto University
|
| Department |
Department of Immunology and Genomic Medicine
|
| Street address |
Yoshida Konoe-cho, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE32224 |
Comparison of gene expression in Wild type and T cell-specific conditional Trim28 KO in TCR stimulated and un-stimulated naive CD4 positive and T regulatory cells |
|
