|
| Status |
Public on Apr 18, 2024 |
| Title |
WT, repeat2, SCRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
peritoneal adipose tissues
|
| Organism |
Mus musculus |
| Characteristics |
tissue: peritoneal adipose tissues
cell type: FALC cells
genotype: Wild-type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Omentum and mesentery adipose tissue were minced in RPMI 1640 Medium (Gibco) containing 2% FBS (Biotechne), 60 μg/ml Liberase TL (millpore sigma), 250 μg/ml DNase I (millpore sigma). Minced adipose tissue was placed at 37°C for 25 minutes, shaking.
Library was performed according to the manufacter's instructions. The final libraries were sequenced with pair-end on Illumina platform with at least 50,000 reads per cell.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation were made using Cell Ranger software v3.0.0(https://support.10xgenomics.com/single-cell-gene-expression/sotware/pipelines/lateset/what-is-cell-ranger)
Assembly: mouse genome mm10
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Dec 19, 2023 |
| Last update date |
Apr 18, 2024 |
| Contact name |
Meihong Deng |
| Organization name |
Feinstein Institutes for Medical Research
|
| Department |
Institute of Molecular Medicine
|
| Street address |
350 community drive
|
| City |
Manhasset |
| State/province |
NY |
| ZIP/Postal code |
11030 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE250571 |
Activation of TLR9 signaling suppresses the immuno modulating functions of CD55lo fibroblastic reticular cells during bacterial peritonitis |
|
| Relations |
| BioSample |
SAMN38926903 |
| SRA |
SRX22963465 |
