|
| Status |
Public on Nov 30, 2011 |
| Title |
MIWI +/- biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
purified Round Spermatids total RNA, adult
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: MIWI +/-
strain: C57BL/6
|
| Treatment protocol |
removal tunica albuginea, collagenase treatment in PBS, cell extraction from seminiferous tubules by pipetting with pasteur pipette, cell fractionation with BSA gradient 2-4%
|
| Growth protocol |
no specific treatment of mice, testes dissection at room temperature, testes kept in cold PBS before cell fractionation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from round spermatids pooled from the respective gradient fractions
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Data processing |
The data were analyzed with RMA from aroma.affy normalization method.
|
| |
|
| Submission date |
Sep 16, 2011 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Ramesh Pillai |
| E-mail(s) |
ramesh.pillai@unige.ch
|
| Organization name |
University of Geneva
|
| Department |
Department of Molecular Biology
|
| Street address |
30, Quai Ernest-Ansermet
|
| City |
Gneveva |
| ZIP/Postal code |
CH-1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE32180 |
MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray] |
| GSE32183 |
MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing |
|
