|
| Status |
Public on Mar 13, 2024 |
| Title |
Pla2g3-/-, antigen challenged, bronchial epithelial cells |
| Sample type |
RNA |
| |
|
| Source name |
Pla2g3-/-, antigen challenged, bronchial epithelial cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bronchial epithelial cells
genotype: Pla2g3-/-
treatment: antigen challenged
|
| Treatment protocol |
3 h after antigen challenge, lungs were harvested, and bronchial epithelial cells were isolated from lung tissues (reference to Kuroishi S et al. Respirology 14, 828-837, 2009) for microarray analysis.
|
| Growth protocol |
Mice were sensitized with intraperitoneal injections of OVA (Grade V, Merk-Sigma-Aldrich) (10 µg) as a antigen plus 1.3 mg of aluminum hydroxide (Alu-Gel-S suspension, SERVA) (alum adjuvant) in 0.2 ml of saline on days 0 and 14. On days 26, 27, and 28, the mice were challenged with 1% OVA aeroallergen for 30 min per day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells and purified, and the quality of RNA was assessed with a 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
200 ng of total RNA was amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
1.65 µg of Cy3-labeled cRNA was fragmented using the Agilent Gene Expression Hybridization Kit and hybridized with a Whole Mouse Genome Microarray (4x44K, Agilent Technologies). Hybridizations were performed for 17 hours at 65ºC at 10 rpm in a rotating hybridization oven.
|
| Scan protocol |
The slides were scanned immediately after washing on SureScan Microarray Scanner (Agilent Technologies) using one color scan setting for 4x44k array slides (Scan resolution 5 um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v11.5.1.1) with set to default for all parameters.
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| |
|
| Submission date |
Dec 01, 2023 |
| Last update date |
Mar 13, 2024 |
| Contact name |
Yoshitaka Taketomi |
| Organization name |
The University of Tokyo
|
| Department |
CDBIM, Graduate School of Medicine,
|
| Lab |
Laboratory of Microenvironmental Metabolic Health Science
|
| Street address |
7-3-1 Hongo, Bunkyo-ku,
|
| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10333 |
| Series (1) |
| GSE249135 |
Effect of group III phospholipase A2 deletion on gene expression in bronchial epithelial cells in an antigen-induced asthma model. |
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