|
| Status |
Public on Jun 01, 2024 |
| Title |
IR_1 |
| Sample type |
SRA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
cell line: CD34+
treatment: IR-2Gy
time: after 2h of IR exposure
|
| Treatment protocol |
TheCD34+ cells were irradiated with 2Gy γ-rays or nonirradiated.
|
| Growth protocol |
The isolated CD34-positive cells were cultured in a Stem Span medium with 5% CO2 at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using TRIzol™ LS Reagent(invitrogen).All the RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using TruePrepTM DNA Library Prep Kit V2 for Illumina® and TruePrepTM Index Kit V2/V3 for Illumina® (vazyme).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
h4_L2_Q0056W0177
|
| Data processing |
NOT PROVIDED; REQUESTED
Assembly: hg38
Supplementary files format and content: tab-delimited text file includes raw counts for each sample
Supplementary files format and content: tab-delimited text file includes RPKM valuses for each sample
|
| |
|
| Submission date |
Nov 26, 2023 |
| Last update date |
Jun 01, 2024 |
| Contact name |
CHENGFANG ZHOU |
| E-mail(s) |
chengfangzhou47@gmail.com
|
| Phone |
(86)13368348471
|
| Organization name |
Army Medical University
|
| Department |
Department of Hematology, Southwest Hospital
|
| Street address |
No. 30, Gaotanyan Street.
|
| City |
Chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE248636 |
Nynrin preserves hematopoietic stem cell function by inhibiting the mitochondrial permeability transition pore opening （human_HSC_RNA_Seq) |
| GSE248639 |
Nynrin preserves hematopoietic stem cell function by inhibiting the mitochondrial permeability transition pore opening. |
|
| Relations |
| BioSample |
SAMN38440419 |
| SRA |
SRX22643571 |
