|
| Status |
Public on Sep 07, 2011 |
| Title |
HEK293 cells was overexpressed the alternative spliced form of lactoferrin gene (ΔLF) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Alternatively spliced form (ΔLF) was overexpressed in HEK293 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293
|
| Growth protocol |
The Flp-In T-Rex human HEK293 cell cultured in DMEM medium with 10% fetal calf serum and penicillin-streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier’s protocols.
|
| Label |
Cy5
|
| Label protocol |
See http://www.phalanxbiotech.com/ for updated protocols
|
| |
|
| Channel 2 |
| Source name |
Isolated total RNA from HEK293 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293
|
| Growth protocol |
The Flp-In T-Rex human HEK293 cell cultured in DMEM medium with 10% fetal calf serum and penicillin-streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier’s protocols.
|
| Label |
Cy3
|
| Label protocol |
See http://www.phalanxbiotech.com/ for updated protocols
|
| |
|
| |
| Hybridization protocol |
See http://www.phalanxbiotech.com/ for updated protocols
|
| Scan protocol |
See http://www.phalanxbiotech.com/ for updated protocols
|
| Data processing |
See http://www.phalanxbiotech.com/ for updated protocols
|
| |
|
| Submission date |
Sep 06, 2011 |
| Last update date |
Sep 07, 2011 |
| Contact name |
Ju Hee Kim |
| Organization name |
Dongguk University
|
| Department |
Life Science
|
| Lab |
Molecular Biology
|
| Street address |
pil-dong 3-ga
|
| City |
seoul |
| ZIP/Postal code |
100-715 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6254 |
| Series (1) |
| GSE31878 |
Gene expression profiling in HEK293 cells overexpressed lactoferrin gene that has the signal sequence (sLF) or not (ΔLF) |
|
