|
| Status |
Public on Nov 05, 2023 |
| Title |
KGN cells, SiSNHG5, rep3 |
| Sample type |
SRA |
| |
|
| Source name |
KGN
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: KGN
cell type: human granulosa-like tumor cell line
genotype: SNHG5 knockdown
treatment: Transfected with siRNA targeting SNHG5
|
| Treatment protocol |
KGN cells were transfected with either siRNA targeting SNHG5 or the negative control siRNA.
|
| Growth protocol |
KGN were cultured in DMEM/F12 containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated at 37 ℃ with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Fourty-eight hours after transfection of either siRNA targeting SNHG5 or the control, KGN cells were harvested and RNA was extracted.1.0 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries were constructed using SMARTER mRNA-seq Library Prep Kit according to the manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using Trimmomatic software
Trimmed sequence reads were mapped to GRch38
Read count extraction and normalization were performed
Assembly: GRCh38
Supplementary files format and content: Text file includes reads counts for each sample
Supplementary files format and content: Text file includes FPKM for each sample
|
| |
|
| Submission date |
Nov 03, 2023 |
| Last update date |
Nov 05, 2023 |
| Contact name |
Zuwei Yang |
| E-mail(s) |
yangzuwei7913@fckyy.org.cn
|
| Organization name |
Obstetrics and Gynecology Hospital, Fudan University
|
| Street address |
No. 419, Fangxie Road
|
| City |
Shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE246995 |
LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis |
|
| Relations |
| BioSample |
SAMN38093831 |
| SRA |
SRX22366529 |
