|
| Status |
Public on Aug 01, 2012 |
| Title |
Line Pair HAD2.LAD2 - Alc Pref NPr - animal L2.11 [ventral tegmental area] |
| Sample type |
RNA |
| |
|
| Source name |
ventral tegmental area from LAD2 rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
line pair: HAD2.LAD2
alcohol preference: NPr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol followed by Qiagen RNeasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 2-cycle protocol from 25 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on RGU34A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the standard Affymetrix protocol.
|
| Description |
Gene expression data from brain ventral tegmental area (VTA)
|
| Data processing |
The data were analyzed with RMA.
|
| |
|
| Submission date |
Aug 26, 2011 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Mark William Kimpel |
| E-mail(s) |
mkimpel@iupui.edu
|
| Organization name |
Indiana University
|
| Department |
Psychiatry
|
| Lab |
W.J. McBride
|
| Street address |
15032 Hunter Court
|
| City |
Westfield |
| State/province |
IN |
| ZIP/Postal code |
46074 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE31695 |
Comparison of Differences in Gene Expression in the Ventral Tegmental Area of 5 Pairs of Rat Lines Selectively Bred for High or Low Alcohol Consumption |
| GSE31709 |
Comparison of Differences in Gene Expression in brain of 5 Pairs of Rat Lines Selectively Bred for High or Low Alcohol Consumption |
|
