|
| Status |
Public on Mar 21, 2012 |
| Title |
V2 (area 18), layer 2; Female 98C096 |
| Sample type |
RNA |
| |
|
| Source name |
V2 (area 18), layer 2; Female 98C096
|
| Organism |
Macaca mulatta |
| Characteristics |
gender: Female
tissue: V2 (area 18)
tissue region: Layer 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from each brain region following the manufacturer's directions for the RNeasy Micro kit. Whole rhesus brain RNA pool obtained from Biochain.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared using 5ng total RNA input and 2 cycles of the GeneChip HT One-Cylce cDNA Synthesis Kit from Affymetrix.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C on the GeneChip Rhesus Macaque Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from the adult primate neocortex.
Macaque_Female 98C096_V2_Layer 2
|
| Data processing |
The data were normalized using Matlab2007b. Each batch was normalized within itself by RMA. Batch effects were subsequently removed by normalizing each probe based on common whole brain control samples across batches. Data is reported as Log10 normalized intensity.
|
| |
|
| Submission date |
Aug 23, 2011 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Susan Sunkin |
| Organization name |
Allen Institute for Brain Science
|
| Street address |
551 N. 34th Street
|
| City |
Seattle |
| ZIP/Postal code |
98103 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE31613 |
Transcriptional architecture of the primate neocortex |
|
