|
| Status |
Public on Apr 07, 2024 |
| Title |
EVs from SEA treated LE/6, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
EVs from SEA treated LE/6 for 24hours
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: LE/6
agent: SEA
|
| Treatment protocol |
LE/6 was treated with SEA (20ug/mL) or PBS for 24hours.
|
| Growth protocol |
Hepatic progenitor cell line, LE/6, were cultured in DMEM with Ham’s F-10 (1:1) supplemented with 1 μg/ml insulin and 0.5 μg/ml hydrocortisone.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell culture medium was harvested every 24 hours from unstimulated or SEA-stimulated HPCs and stored at -80℃ until isolation of EVs. HPC-conditioned medium (CM) and serum were centrifuged for 10 minutes at 300g, 10minutes at 2000g and 30 minutes at 10000g at 4℃ to remove cell debris and aggregates. Supernatant was transferred into new tubes and ultracentrifuged at 120000g for 2.5 hours by Beckman. EVs were resuspended in PBS in equal volumes for miRNA array. Trizol extraction of total miRNA was performed according to the manufacturer's protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
Gene expresssion data in EVs from SEA treated LE/6
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 20, 2023 |
| Last update date |
Apr 07, 2024 |
| Contact name |
YUE YUAN |
| E-mail(s) |
D202181938@hust.edu.cn
|
| Phone |
+8615521257192
|
| Organization name |
tongji medical college
|
| Street address |
none
|
| City |
Wu Han |
| ZIP/Postal code |
430000 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
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