|
| Status |
Public on Apr 24, 2024 |
| Title |
Poly(I:C) + artLCMV-E7E6 treated tumor biological replicate 2 DAY7 |
| Sample type |
SRA |
| |
|
| Source name |
Tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Tumor
tag: Poly(I:C) + artLCMV-E7E6 treated
strain: C57BL/6
genotype: ID8.OVA.Vegf/GFP
|
| Treatment protocol |
Seven days later, mice were intravenously treated with 1×105 RCV FFU artLCMV-E7E6 and received intratumoral injections of 50 μg of poly(I:C) in the right-sided tumor lesion on days 7, 10, and 13.
|
| Growth protocol |
Mice were subcutaneously injected with 1.5×105 TC-1/A9 cells in the right flank. Seven days later, tumor were treated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
On day 8 and day 14, mice were euthanized, and RNA from tumors was subjected to RNAseq analysis
TruSeq Stranded mRNA preparation
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
d7E7PlyR3
NovaSeq6000 150PE (150 x 2bp) 80M total reads 12Gb/sample
|
| Data processing |
FastQC for quality control
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm39 using STAR
Quantification using FeatureCounts
Assembly: mm39
Supplementary files format and content: tab-delimited text files include log2CPM values for each Sample in day1 and day7
|
| |
|
| Submission date |
Oct 16, 2023 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Jose Gonzalez |
| E-mail(s) |
jgonzalezgom@unav.es
|
| Organization name |
FIMA
|
| Street address |
C/PIO XII s/n
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE245436 |
The efficacy of LCMV-based cancer vaccines is unleashed by intratumoral injections of polyI:C |
|
| Relations |
| BioSample |
SAMN37831868 |
| SRA |
SRX22099993 |
