|
| Status |
Public on Oct 11, 2023 |
| Title |
MoS2_2 |
| Sample type |
SRA |
| |
|
| Source name |
Mouse breast cancer cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: 4T1
cell states: Normal culture condition
intervention: MoS2
|
| Treatment protocol |
cell cultured with medium, MoS2 and MoS2+NIR
|
| Growth protocol |
10% FBS, 1% penicillin/streptomycin, high glucose DMEM
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
About 10e6 4T1 cells were isolated from different group and the mRNA library constructed using QIAseq FX Single Cell RNA Library Kit (QIAGEN) according to the manufacturer’s instruction
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie v0.12.9.
Fragments Per Kilobase of exon model per Million mapped fragments (FPKM) was performed with Cufflinks v2.1.1, aligned reads were counted with HTSeq.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Oct 09, 2023 |
| Last update date |
Oct 11, 2023 |
| Contact name |
ke li |
| E-mail(s) |
llllike@alu.cqu.edu.cn
|
| Phone |
18002399439
|
| Organization name |
chongqing university
|
| Street address |
shazheng street 174
|
| City |
chongqing |
| ZIP/Postal code |
400030 |
| Country |
China |
| |
|
| Platform ID |
GPL14844 |
| Series (1) |
| GSE244922 |
All-in-one Engineering Multifunctional Nanoplatforms for Sensitizing Tumor Low-Temperature Photothermal Therapy in vivo |
|
| Relations |
| BioSample |
SAMN37735360 |
| SRA |
SRX22036160 |
