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| Status |
Public on Feb 04, 2025 |
| Title |
Drosophila heads, repo-Gal4 control, sample 3 |
| Sample type |
SRA |
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| Source name |
adult heads
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| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: adult heads
genotype: repo-Gal4/+
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Micro Kit (Qiagen) according to protocol (neuronal Bchs overexpression dataset) or exchanging the first step of homogenization in RLT buffer with the following Trizol protocol (glial Bchs overexpression dataset). Heads were homogenized in 500 µl Trizol, centrifuged for 5 min at 10000 rpm at 4°C, supernatant was tranferred to a new tube, 200 µl Chloroform (Carl Roth, No. 6340.1) was added and the tube was shaked. An incubation step at room temperature for 3 min was followed by centrifugation for 5 min at 10,000 rpm at 4°C. The upper aqueous phase was transferred in a new tube and it was continued with the RNeasy Micro Kit protocol.
RNA-seq libraries were prepared using TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
STAR,HTSEQ
Assembly: BDGP6.46 (GCA_000001215.4)
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| Submission date |
Oct 05, 2023 |
| Last update date |
Feb 04, 2025 |
| Contact name |
Diana Le Duc |
| Organization name |
Institute of Human Genetics,University of Leipzig Medical Center
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| Street address |
Philipp-Rosenthal-Str. 55
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| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platform ID |
GPL25244 |
| Series (1) |
| GSE244775 |
Drosophila Bchs overexpression recapitulates human WDFY3 neurodevelopmental phenotypes with implications for glial cell involvement in altered head circumference |
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| Relations |
| BioSample |
SAMN37699350 |
| SRA |
SRX22005638 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7829515_MK_3_TR44xw1118_S8.txt.gz |
88.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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