|
| Status |
Public on Aug 16, 2013 |
| Title |
IL28b-1 |
| Sample type |
RNA |
| |
|
| Source name |
IL28b-1
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: IL28b
tissue: liver
cell type: hepatocytes
|
| Treatment protocol |
RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
|
| Growth protocol |
Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
RMA nomanization method of software Partek
|
| |
|
| Submission date |
Aug 18, 2011 |
| Last update date |
Aug 16, 2013 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE31455 |
Primary human hepatocytes treated with IL28B and the HCV JFH1 Genotype 2A clone. |
|
