|
| Status |
Public on Aug 18, 2011 |
| Title |
MCF7 cells after 72hr treatment with 5uM 5-Aza-2'-deoxycytidine(Aza) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
treatment with 5uM 5-Aza-2'-deoxycytidine(Aza)
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: 5uM 5-Aza-2'-deoxycytidine for 72 h
cell line: MCF7
|
| Treatment protocol |
A human breast cancer cell line, MCF-7, was treated with 5-Aza-2'-deoxycytidine(Aza), at 5μM and 10μM for 72hr. Culture medium with or without treatment was changed every 24 hr.
|
| Growth protocol |
A human breast cancer cell line, MCF-7, was grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier's protocols.
|
| Label |
Cy3
|
| Label protocol |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| |
|
| Channel 2 |
| Source name |
untreated
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: untreated
cell line: MCF7
|
| Treatment protocol |
A human breast cancer cell line, MCF-7, was treated with 5-Aza-2'-deoxycytidine(Aza), at 5μM and 10μM for 72hr. Culture medium with or without treatment was changed every 24 hr.
|
| Growth protocol |
A human breast cancer cell line, MCF-7, was grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier's protocols.
|
| Label |
Cy5
|
| Label protocol |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| |
|
| |
| Hybridization protocol |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| Scan protocol |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| Description |
Analysis used aerobic steady state RNA as control samples for comparison to the experimental samples taken at 5, 10, 15 and 60 min.
|
| Data processing |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| |
|
| Submission date |
Aug 17, 2011 |
| Last update date |
Aug 18, 2011 |
| Contact name |
Ju Hee Kim |
| Organization name |
Dongguk University
|
| Department |
Life Science
|
| Lab |
Molecular Biology
|
| Street address |
pil-dong 3-ga
|
| City |
seoul |
| ZIP/Postal code |
100-715 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6254 |
| Series (1) |
| GSE31441 |
MCF7 cells gene expression change by inducing CpG demethylation using 5-Aza-2'-deoxycytidine (Aza) |
|
