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| Status |
Public on Jan 11, 2024 |
| Title |
DRG 5 |
| Sample type |
SRA |
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| Source name |
DRG
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| Organism |
Homo sapiens |
| Characteristics |
tissue: DRG
age: 49 years old
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Samples were immediately cleaned from the blood and connective tissue and frozen on dry ice.The whole ganglia were embedded in OCT for spatial transcriptomics.Cryosections were cut at 10-μm thickness and mounted onto the GEX arrays. Sections were placed on a Thermocycler Adaptor with the active surface facing up and were incubated at 37°C for 1 min. Then, sections were fixed with methyl alcohol at -20°C for 30 min, followed by staining with hematoxylin and eosin (H&E) (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702).
Visum spatial libraries were constructed using Visum spatial Library construction kit (10x Genomics, PN-1000184) according to the manufacture’s introduction.
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| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Basecalls performed using bcl2fastq tools
Visum spatial gene expression was processed using Visum spatial gene expression slide and Reagent Kit (10x Genomics, PN-1000184).
Assembly: GRCh38
Supplementary files format and content: Tab separated values files with barcode sequences corresponding to column indices, features corresponding to row indices
Library strategy: spatial transcriptomics
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| Submission date |
Sep 13, 2023 |
| Last update date |
Jan 11, 2024 |
| Contact name |
Donghang Zhang |
| Organization name |
West China Hospital of Sichuan University
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| Street address |
No.37, guoxue lane, wuhou district, chengdu city, sichuan province
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (2) |
| GSE243073 |
Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord [DRG Spatial] |
| GSE243077 |
Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord |
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| Relations |
| BioSample |
SAMN37381919 |
| SRA |
SRX21766807 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7778671_DRG5_filtered_feature_bc_matrix.tar.gz |
13.6 Mb |
(ftp)(http) |
TAR |
| GSM7778671_DRG_5_ch00.jpg.gz |
32.7 Mb |
(ftp)(http) |
JPG |
SRA Run Selector |
| Raw data are available in SRA |
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