|
| Status |
Public on Jan 01, 2024 |
| Title |
WYKNIU2 |
| Sample type |
SRA |
| |
|
| Source name |
scraped urothelium
|
| Organism |
Mus musculus |
| Characteristics |
tissue: scraped urothelium
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single cell suspensions were generated using the Miltenyi Biotec Tumor Dissociation Kit and the gentleMACS Dissociator. Red blood cells were lysed using Miltenyi Biotec Red Blood Cell Lysis Solution and dead cells were removed using the Miltenyi Biotec Dead Cell Removal Kit. Single cell suspensions were subsequently loaded onto the 10X Genomics Chromium Controller to generate single-cell gel beads in emulsions (GEMs)
scRNAseq libraries were prepared using Chromium Single Cell 3' Reagent Kits (v3.1 Chemistry) according to the manufacter's instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting were made using the Cell Ranger software v6.1.1
Assembly: mm10
Supplementary files format and content: H5 format files of cell ranger output for each scRNAseq samples
|
| |
|
| Submission date |
Sep 06, 2023 |
| Last update date |
Jan 01, 2024 |
| Contact name |
William Kim |
| E-mail(s) |
william_kim@med.unc.edu
|
| Organization name |
UNC Chapel Hill
|
| Department |
Lineberger Comprehensive Cancer Center
|
| Street address |
450 West Dr
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE217093 |
RNAseq and scRNAseq analysis of UPFL mouse tumors |
|
| Relations |
| BioSample |
SAMN37300245 |
| SRA |
SRX21646651 |
