|
| Status |
Public on Sep 28, 2024 |
| Title |
scOVCA3_CD45 |
| Sample type |
SRA |
| |
|
| Source name |
ovarian cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: ovarian cancer
cell line: primary cell
cell type: CD45+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumor specimens were dissociated, stained and FACS sorted for FVD- CD45+. Isolated cells were washed with 0.5% BSA/PBS twice subjected to single-cell transcriptomic analysis using Chromium 10X platform
scRNA-seq libraries were generated using Chromium 10x 3' v3.1 kit following manufacturer's protocol and sequenced by Illumina NovaSeq6000
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Chromium 10x Single Cells 3' v3.1
Metadata.csv
|
| Data processing |
fastq files were aligned to GRCh38-2020-A_build reference genome from 10x Genomics using Cell Ranger
Assembly: GRCh38-2020-A_build (10x Genomics)
Supplementary files format and content: tar archive of CellRanger output files: barcodes.tsv.gz, features.tsv.gz, matrix.mtx.gz
Supplementary files format and content: Cell type annotation file: Metadata.csv
|
| |
|
| Submission date |
Sep 05, 2023 |
| Last update date |
Sep 28, 2024 |
| Contact name |
Shuh Narumiya |
| E-mail(s) |
snaru@mfour.med.kyoto-u.ac.jp
|
| Organization name |
Kyoto University
|
| Department |
Department of Drug Discovery Medicine
|
| Lab |
Narumiya-G
|
| Street address |
Yoshidahonmachi
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE242271 |
Prostaglandin E2-EP4 signaling shapes immunosuppressive tumor microenvironment in human tumors by suppressing bioenergetics and ribosome biogenesis in infiltrating immune cells [scRNA-seq] |
|
| Relations |
| BioSample |
SAMN37279957 |
| SRA |
SRX21633543 |
