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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 04, 2023 |
| Title |
MEF |
| Sample type |
RNA |
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| Source name |
Mouse embryonic fibroblast
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| Organism |
Mus musculus |
| Characteristics |
cell type: Fibroblast
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| Extracted molecule |
total RNA |
| Extraction protocol |
Using RNeasy and miRNeasy Mini Kits (Qiagen, Germany) following the supplier's instructions. Catalog number #RA670A-1
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| Label |
SYBR Green
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| Label protocol |
Mouse miRNome Sanger miRBase micro RNA Profiling Set ( Sytem Biosciences, USA) following the Manufacturer’s instructions. Using 10pg-10ug total RNA, tag all small RNAs with poly-A tails, anneal an oligo-dT adaptor, and generate first-strand cDNA through reverse transcription. Quantitative real-time PCR were performed with 40 cycles at 50 oC for 2minutes, 95 oC for 10minutes, 95 oC for 15 seconds and 60 oC for 1minutes.
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Description |
control
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| Data processing |
miRNA microarray data were normalized by logarithmic transformation
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| Submission date |
Aug 30, 2023 |
| Last update date |
Sep 04, 2023 |
| Contact name |
Kinarm Ko |
| E-mail(s) |
knko@kku.ac.kr
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| Organization name |
Konkuk University
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| Street address |
120, Neungdong-ro, Gwangjin-gu
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| City |
Seoul |
| ZIP/Postal code |
05029 |
| Country |
South Korea |
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| Platform ID |
GPL29570 |
| Series (1) |
| GSE241920 |
Quantitative PCR (qRT-PCR) analysis of micro RNA expression in mouse cells |
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| Supplementary data files not provided |
| Processed data are available on Series record |
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