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| Status |
Public on Mar 08, 2024 |
| Title |
NeoT_1, biological replicate 1, WGS |
| Sample type |
SRA |
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| Source name |
cell line
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| Organism |
Homo sapiens |
| Characteristics |
cell line: cell line
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was prepared via the novel ProMTag Multiomics workflow described in the manuscript. The DNA was treated with RNase A before being sent to Novogene for WGS.
Genomic DNA was sheared into 350 bp fragments and libraries were constructed using the NEBNext DNA Library Prep Kit, followed by end repair, dA-tailing, and ligation with NEBNext adapter. Fragments (300-500 bp) were PCR enriched by p5 and indexed P7 oligos.
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| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
DNA sequencing files were processed using the nf-core/sarek pipeline (v3.0.1)
Assembly: GRCh38
Supplementary files format and content: processed data are provided in vcf files which include the unfiltered variants in each of the cell lines compared to the control cell line (A10).
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| Submission date |
Aug 22, 2023 |
| Last update date |
Mar 08, 2024 |
| Contact name |
Stephanie D Byrum |
| Organization name |
University of Arkansas for Medical Sciences
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| Street address |
501 Jack Stephens Dr, Jackson T. Stephens Bldg 4th floor
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| City |
Little Rock |
| State/province |
Arkansas |
| ZIP/Postal code |
72205 |
| Country |
USA |
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| Platform ID |
GPL24676 |
| Series (2) |
| GSE241433 |
One-pot method for preparing DNA, RNA, and protein for multiomics analysis II |
| GSE241434 |
One-pot method for preparing DNA, RNA, and protein for multiomics analysis |
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| Relations |
| BioSample |
SAMN37116168 |
| SRA |
SRX21457625 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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