|
| Status |
Public on Dec 05, 2023 |
| Title |
Male Hippocampus_dflox control_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, dflox control
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL/6
genotype: dflox control
tissue: hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA for microarray analysis was isolated from the whole hippocampus of adult male and female dflox control and GREmx1-cre, MREmx1-cre, and GRMREmx1-cre mice using the RNeasy Mini Kit (Qiagen) and RNase-Free DNase Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Gene expression from male hippocampus, dflox control
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v12.0), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Aug 15, 2023 |
| Last update date |
Dec 05, 2023 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE240873 |
Glucocorticoid and Mineralocorticoid Receptor Signaling have Sex-dependent and Independent Effects in Mouse Hippocampus |
|
