|
| Status |
Public on Apr 01, 2016 |
| Title |
Untreated nasal cells from smoker volunteer 2 |
| Sample type |
RNA |
| |
|
| Source name |
Untreated primary human nasal epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary human nasal epithelial cells
|
| Treatment protocol |
Expanded cells were mock-treated with the vehicle control, dimethyl sulfoxide, for 6 hours
|
| Growth protocol |
Human nasal epithelial cells were extracted from healthy smoking volunteers and cultured in bronchial epithelial growth medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction protocol
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on Affymetrix Human Gene 1.0 ST array
|
| Scan protocol |
GeneChips were scanned using Affymetrix scanner
|
| Description |
Gene expression data from untreated nasal epithelial cells
|
| Data processing |
Data were analyzed with robust multichip analysis in Partek® Genomics SuiteTM software (version 6.5)
|
| |
|
| Submission date |
Jul 27, 2011 |
| Last update date |
Apr 01, 2016 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE30982 |
Primary human nasal cells extracted from current smokers treated with kaempferol |
|
