|
| Status |
Public on Aug 31, 2012 |
| Title |
E2f3a+/+;E2f7+/+;E2f8+/+ embryo WT 6 |
| Sample type |
RNA |
| |
|
| Source name |
Embryo E10.5
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Embryo
age: E10.5
genotype: E2f3a+/+;E2f7+/+;E2f8+/+
genetic background: mixed 129SvEv, C57BL/6NTac and FVB/Ntac
|
| Treatment protocol |
Embryos and placentas were collected from timed pregnancies at 10.5 days post coitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430.2 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Gene Chips were scanned using the Affy GeneChip Scanner 3000.
|
| Description |
Embryo E10.5 Gene expression data
|
| Data processing |
The data were analyzed with RMAExpress (version 1.0 beta 4) and normalized using Quantile Normalization. Robust mean analysis (RMA) was performed with default parameter settings and the output was log transformed.
|
| |
|
| Submission date |
Jul 07, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Madhu Ouseph |
| E-mail(s) |
madhu.ouseph@osumc.edu
|
| Phone |
6142922459
|
| Organization name |
The Ohio State University
|
| Street address |
460w 12th Ave
|
| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE30488 |
Expression data from E2f7/E2f8/E2f3a null placentas and embryos |
|
