|
| Status |
Public on Sep 05, 2023 |
| Title |
Hypothalamus_P-group_4 |
| Sample type |
RNA |
| |
|
| Source name |
Hypothalamus_powder diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
age: 4 weeks old
diet: powder chow
|
| Treatment protocol |
Rats were administered powdered or solid feed for 8 days.
|
| Growth protocol |
Rats were housed under controlled conditions at a temperature of 20–26°C and humidity of 40–60% under a 12/12-h light-dark cycle, with ad libitum feed and water intake.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the hypothalamus sample using TRIzol Reagent(Thermo Fisher Scientific) , according to the manufacture's protocol. The extracted total RNA was cleaned up with RNeasy Mini Kit (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was synthesized from 100ng total RNA according to the manufacture's protocol.
|
| |
|
| Hybridization protocol |
cRNA 15µg were flagmentated, and hybridized for 16hr at 45 on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained with Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
The data were normalized using the statistical software R, and the qFARMS method.
|
| |
|
| Submission date |
Jul 03, 2023 |
| Last update date |
Sep 05, 2023 |
| Contact name |
yoshikazu saito |
| E-mail(s) |
mokichi31101003@gmail.com
|
| Phone |
+81-35841-1879
|
| Organization name |
university of tokyo
|
| Street address |
1-1-1
|
| City |
bunkyo |
| State/province |
tokyo |
| ZIP/Postal code |
1138657 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE236446 |
Mastication stimuli regulate the heart beat rate through the rhythmic regulation by hypothalamic-autonomic system in the weaning stage rat |
|
