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Sample GSM749747 Query DataSets for GSM749747
Status Public on Jun 29, 2011
Title Stam_NHEK_CTCF_2
Sample type SRA
 
Source name NHEK
Organism Homo sapiens
Characteristics cell: NHEK
cell organism: Human
cell description: epidermal keratinocytes
cell karyotype: normal
cell lineage: ectoderm
cell sex: F
antibody: CTCF
antibody description: rabbit polyclonal
antibody target description: CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.
antibody vendor name: Millipore
replicate: 2
Biomaterial provider Lonza
Growth protocol Cells were grown according to ENCODE cell culture protocols: http://genome.ucsc.edu/ENCODE/protocols/cells/human/Keratinocyte_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol For extraction protocol details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeUwTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
 
Data processing For data processing details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeUwTfbs
 
Submission date Jun 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9115
Series (2)
GSE30263 CTCF Binding Sites by ChIP-seq from ENCODE/University of Washington
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX080410
BioSample SAMN00631427
Named Annotation GSM749747_hg19_wgEncodeUwTfbsNhekCtcfStdRawRep2.bigWig

Supplementary file Size Download File type/resource
GSM749747_hg19_wgEncodeUwTfbsNhekCtcfStdAlnRep2.bam 414.4 Mb (ftp)(http) BAM
GSM749747_hg19_wgEncodeUwTfbsNhekCtcfStdHotspotsRep2.broadPeak.gz 1.7 Mb (ftp)(http) BROADPEAK
GSM749747_hg19_wgEncodeUwTfbsNhekCtcfStdPkRep2.narrowPeak.gz 682.3 Kb (ftp)(http) NARROWPEAK
GSM749747_hg19_wgEncodeUwTfbsNhekCtcfStdRawRep2.bigWig 72.0 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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