|
| Status |
Public on Dec 29, 2011 |
| Title |
Liver from db/db F2 mouse at 5 weeks of age [125] |
| Sample type |
RNA |
| |
|
| Source name |
F2 mouse at 5 weeks
|
| Organism |
Mus musculus |
| Characteristics |
strain: B57BL/6 X DBA/2
gender: Male
age: 5 weeks
plasma glucose mg per dl: 222
insulin uiu per ml: 31.3
trglycerides mg per dl: 81
total chol mg per dl: 175
hdl chol mg per dl: 135
unesterified chol mg per dl: 50
free fatty acids mg per dl: 51
bodyweight g: 25.4
nmr fat: 10.39
nmr lean: 18.91
nmr %fat: 35.07
gonadal g: 1.02
retroperitoneal g: 0.1
mesenteric g: 0.14
subcutaneous g: 0.75
livernmrfat g: 0.102
livernmrlean g: 0.606
livernmr%fat: 0.144
|
| Treatment protocol |
Chow diet
|
| Growth protocol |
F2 mice, group housed until 5 weeks or 12 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver biopsy samples were snap frozen and stored at -80oC. Total RNA was extracted from the tissue using Trizol reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
@52002900776324092709406245176183.CEL
|
| Data processing |
Expression measurements were background-adjusted, normalized, and summarized with RMA implemented in R
|
| |
|
| Submission date |
Jun 22, 2011 |
| Last update date |
Dec 29, 2011 |
| Contact name |
Calvin Pan |
| Organization name |
UCLA
|
| Street address |
675 Charles Young Dr South
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE30140 |
Expression data from livers of F2 mice (C57BL/6 X DBA/2) deficient in leptin receptor (db/db) |
|
