|
Status |
Public on Dec 29, 2011 |
Title |
Liver from db/db F2 mouse at 5 weeks of age [51] |
Sample type |
RNA |
|
|
Source name |
F2 mouse at 5 weeks
|
Organism |
Mus musculus |
Characteristics |
strain: B57BL/6 X DBA/2 gender: Male age: 5 weeks plasma glucose mg per dl: 163 insulin uiu per ml: 33 trglycerides mg per dl: 72 total chol mg per dl: 123 hdl chol mg per dl: 92 unesterified chol mg per dl: 38 free fatty acids mg per dl: 21 bodyweight g: 20.3 nmr fat: 6.42 nmr lean: 15.18 nmr %fat: 29.46 gonadal g: 0.201 retroperitoneal g: 0.088 mesenteric g: 0.103 subcutaneous g: 0.402 livernmrfat g: 0.077 livernmrlean g: 0.507 livernmr%fat: 0.132
|
Treatment protocol |
Chow diet
|
Growth protocol |
F2 mice, group housed until 5 weeks or 12 weeks of age
|
Extracted molecule |
total RNA |
Extraction protocol |
Liver biopsy samples were snap frozen and stored at -80oC. Total RNA was extracted from the tissue using Trizol reagent (Invitrogen).
|
Label |
biotin
|
Label protocol |
Standard Affymetrix procedures
|
|
|
Hybridization protocol |
Standard Affymetrix procedures
|
Scan protocol |
Standard Affymetrix procedures
|
Description |
@52002900769113070209405773904931.CEL
|
Data processing |
Expression measurements were background-adjusted, normalized, and summarized with RMA implemented in R
|
|
|
Submission date |
Jun 22, 2011 |
Last update date |
Dec 29, 2011 |
Contact name |
Calvin Pan |
Organization name |
UCLA
|
Street address |
675 Charles Young Dr South
|
City |
Los Angeles |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE30140 |
Expression data from livers of F2 mice (C57BL/6 X DBA/2) deficient in leptin receptor (db/db) |
|