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Sample GSM745918 Query DataSets for GSM745918
Status Public on Sep 02, 2013
Title Liver_WT_rep1
Sample type RNA
 
Source name Liver
Organism Mus musculus
Characteristics strain: mixed background 129sv and C57Bl/6
tissue: liver
genotype/variation: WT
Extracted molecule total RNA
Extraction protocol Standard single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Label Cyanine 3-CTP
Label protocol Standard Agilent protocol that uses T7 RNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3-labeled CTP (Manual:One-Color Microarray-Based Gene Expression Analysis)
 
Hybridization protocol Standard Agilent protocol using suggested blocking agent, fragmentation buffer and hybridization buffer. Hybridization run for 17 hours at 65°C. (Manual:One-Color Microarray-Based Gene Expression Analysis)
Scan protocol Scanning was performed using Agilnet scanner at 5 uM resolution, green dye channel
Data processing Signal was extracted using feature expression and data were nornalized to the 75th percentile of of all of non-control probes on the microarray using genespring software
 
Submission date Jun 21, 2011
Last update date Sep 02, 2013
Contact name Alessandro Provenzani
E-mail(s) alessandro.provenzani@unitn.it
Phone +390461283094
Organization name University of Trento
Department CIBIO
Lab Laboratory of Genomic Screening
Street address via delle regole 101
City Trento
ZIP/Postal code 38100
Country Italy
 
Platform ID GPL7202
Series (1)
GSE30139 Role of Per2 in the control of liver glycogen metabolism

Data table header descriptions
ID_REF
RAW Raw data
VALUE Normalized data
PMA Present/Marginal/Absent

Data table
ID_REF RAW VALUE PMA
GE_BrightCorner 36203.625 -0.43265676 P
DarkCorner 13.242667 0.659987 A
A_52_P616356 8.776502 -0.2835617 A
A_52_P580582 34.05206 -0.35388422 P
A_52_P403405 8.815689 -1.2925029 A
A_52_P819156 8.833006 -1.1789026 A
A_51_P331831 147.17525 -0.22865343 P
A_51_P430630 8.878952 -0.327919 A
A_52_P502357 8.892614 0.062337875 A
A_52_P299964 8.904675 -0.46675205 A
A_51_P356389 8.918072 -0.64696646 A
A_52_P684402 538.2057 -0.008041859 P
A_51_P414208 111.92621 0.6476908 P
A_51_P280918 1018.5297 0.0998044 P
A_52_P613688 216.63304 2.70292 P
A_52_P258194 299.33408 3.6182098 P
A_52_P229271 4221.7847 5.271824 A
A_52_P214630 117.05005 -0.22218895 P
A_52_P579519 610.36145 0.9254174 P
A_52_P979997 306.95102 4.8268924 P

Total number of rows: 41252

Table truncated, full table size 1447 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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