|
| Status |
Public on Sep 02, 2013 |
| Title |
Liver_mPER2_KO_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed background 129sv and C57Bl/6
tissue: liver
genotype/variation: mper2brdm1 mutant (PMID: 10408444)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
|
| Label |
Cyanine 3-CTP
|
| Label protocol |
Standard Agilent protocol that uses T7 RNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3-labeled CTP (Manual:One-Color Microarray-Based Gene Expression Analysis)
|
| |
|
| Hybridization protocol |
Standard Agilent protocol using suggested blocking agent, fragmentation buffer and hybridization buffer. Hybridization run for 17 hours at 65°C. (Manual:One-Color Microarray-Based Gene Expression Analysis)
|
| Scan protocol |
Scanning was performed using Agilnet scanner at 5 uM resolution, green dye channel
|
| Data processing |
Signal was extracted using feature expression and data were nornalized to the 75th percentile of of all of non-control probes on the microarray using genespring software
|
| |
|
| Submission date |
Jun 21, 2011 |
| Last update date |
Sep 02, 2013 |
| Contact name |
Alessandro Provenzani |
| E-mail(s) |
alessandro.provenzani@unitn.it
|
| Phone |
+390461283094
|
| Organization name |
University of Trento
|
| Department |
CIBIO
|
| Lab |
Laboratory of Genomic Screening
|
| Street address |
via delle regole 101
|
| City |
Trento |
| ZIP/Postal code |
38100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE30139 |
Role of Per2 in the control of liver glycogen metabolism |
|
