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Sample GSM743699 Query DataSets for GSM743699
Status Public on Dec 25, 2011
Title CC_ADSV49F.P..1._8.2010 [HuGene-1_0-st]
Sample type RNA
 
Source name ADSV_49_derived_MPCs_+PPAR2_rep1
Organism Homo sapiens
Characteristics cell type: MPC
agent: PPAR2
Extracted molecule total RNA
Extraction protocol Total RNA from human cell lines or human fat was extracted with Trizol (Invitrogen) and purified via the RNeasy mini kit (Qiagen) according to the manufacturers’ instructions. RNA quality was assessed using an Aglient Bioanalyzer
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description Gene Expression
MINTY_p_Cowen_Affy1.0ST_plate_HuGene-1_0-st-v1.r4_C01_646928.CEL
CC_ADSV49F(P)(1)_8-2010
Data processing Data were processed using the BioConductor affy package in R. RMA background correction was applied to probe intensities followed by quantile normalization. Probe set intensities were summarized from individual probes (excluding mismatch probes) using median polished. Gene level intensities were generated by mapping probe sets to refseq annotations. Probe sets that did not map to refseq annotations were not considered in further analyses. Probe Sets mapping to the same genes were median collapsed by gene symbol. Final normalized data was generated by median centering each array after the prior processing.
probe group file: [Exon array] probe group file: HuGene-1_0-st-v1.r4.pgf/HG-U133_Plus_2.pgf
meta-probeset file: [Exon array] meta-probeset file: HuGene-1_0-st-v1.r4.mps/HG-U133_Plus_2.mps
 
Submission date Jun 16, 2011
Last update date Dec 25, 2011
Contact name Tim D Ahfeldt
E-mail(s) tim.ahfeldt@mssm.edu
Organization name Icahn School of Medicine at Mount Sinai
Department Neuroscience
Lab Tim D Ahfeldt
Street address 1425 Madison Ave
City NYC
State/province NY
ZIP/Postal code 10029
Country USA
 
Platform ID GPL6244
Series (2)
GSE30038 Programming human pluripotent stem cells into adipocytes [Affymetrix]
GSE30041 Programming human pluripotent stem cells into adipocytes

Data table header descriptions
ID_REF
VALUE Quantile normalized, median centered(array) gene level expression values from Bioconductor affy package

Data table
ID_REF VALUE
7892501 9.873684646
7892502 40.09541576
7892503 14.44990467
7892504 454.2619084
7892505 22.4885417
7892506 37.68988795
7892507 43.36854956
7892508 29.4084447
7892509 858.637172
7892510 16.18091126
7892511 17.24467644
7892512 124.7657335
7892513 26.504926
7892514 1102.262545
7892515 1435.416668
7892516 10.00299935
7892517 30.25084163
7892518 19.42653724
7892519 32.42455111
7892520 801.6251828

Total number of rows: 32321

Table truncated, full table size 627 Kbytes.




Supplementary file Size Download File type/resource
GSM743699.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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