|
| Status |
Public on Jun 28, 2023 |
| Title |
F4R1Jan2018 |
| Sample type |
SRA |
| |
|
| Source name |
spleen B cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen B cells
disease state: VDJ-hCu
timepoint: Day 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (ThermoFisher) from 5 millions of CD43- spleen B cells
For each sample, 500 ng total RNA was reverse-transcribed to cDNA by a 5’RACE PCR using ProtoScript II (New England Biolabs) and a cap race primer associated with consensus reverse primers for each mouse IgH Cm, Cg and Ca CH1 region or the human Cµ CH1
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
RACE |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Fastq files (R1, R2) were assembled using FLAsH (Fast Length Adjustment of SHort reads) version 1.2.11
Annotation of the consensus with the IMGT HighV-QUEST tools v3.6.0. Parameters : Locus:IGH, species: Mus muris, other parameters as default values.
Supplementary files format and content: IMGT HighV-QUEST output archive, containing tabular files
|
| |
|
| Submission date |
May 25, 2023 |
| Last update date |
Jun 28, 2023 |
| Contact name |
Delphine Rossille |
| E-mail(s) |
delphine.rossille@univ-rennes1.fr
|
| Organization name |
INSERM U1236-MOBIDIC
|
| Street address |
2 avenue du Pr. Léon Bernard
|
| City |
Rennes |
| ZIP/Postal code |
35000 |
| Country |
France |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE233503 |
Immunoglobulin repertoires in mice carrying a knock-in human Cµ gene downstream of the IGH 3' regulatory region |
|
| Relations |
| BioSample |
SAMN35369342 |
| SRA |
SRX20519082 |
