|
| Status |
Public on Nov 13, 2023 |
| Title |
CT26 POLE-mutant+PBS, M44 |
| Sample type |
SRA |
| |
|
| Source name |
CT26 POLE-mutant+PBS
|
| Organism |
Mus musculus |
| Characteristics |
tissue: CT26 tumor
genotype: POLE-mutant
treatment: PBS
|
| Treatment protocol |
/
|
| Growth protocol |
/
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of tumor tissues was harvested using Trizol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The raw data was checked by FastQC
Clean reads were aligned to the mouse genome using STAR 2.5
The expression of the transcripts was calculated by FPKM using Cuffnorm
Differentially expression transcripts were determined using DESeq
Assembly: mm10
Supplementary files format and content: FPKM values for each Sample
|
| |
|
| Submission date |
May 22, 2023 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Pingping Xu |
| E-mail(s) |
xupingpingapple@sina.cn
|
| Organization name |
Shanghai Institute of Digestive Diseases, Renji Hospital, Shanghai Jiao-Tong University School of Medicine
|
| Street address |
145 Middle Shandong Road
|
| City |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE233162 |
Mutations in CHEK2 affect the tumor immune microenvironment and increase the response to PD-1 inhibitor therapy |
|
| Relations |
| BioSample |
SAMN35340779 |
| SRA |
SRX20489433 |
