DNA was extracted from blood or mouthwash sample using standard protocols.
Label
Biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Scan protocol
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
Description
saliva Hybridized to 6.0 chip
Data processing
CEL files were processed using Birdseed version 2 in the Affymetrics Power Tools package. Standard genotype calls with missing data or no call represented by blanks (NN). Important note: Calls were based on an early version of the Affymetrix annotation file. Therefore some AT and GC SNPs will be strand reversed relative to the current hg19 build.
