|
| Status |
Public on Jun 25, 2024 |
| Title |
S1_OCT4-mCherry nEnd, TSC |
| Sample type |
SRA |
| |
|
| Source name |
OCT4-mCherry
|
| Organism |
Mus musculus |
| Characteristics |
cell line: OCT4-mCherry
cell type: Differentiated nEnd
treatment: TSC differentiation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples isolated by fluorescence activated cell sorting (FACS) based on DAPI negative cells using a Sony SH800Z flow cytometer.
CellPlex
single cell RNA-seq for gene expression quantification
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Data processing |
The preprocessing was carried out by following CellPlex cellranger workflow.
Raw reads where demultiplexed and converted to FASTQ using cellranger mkfastq.
Reads were further demultiplexed and counted using cellranger multi command.
Assembly: mm10-2020-A
|
| |
|
| Submission date |
May 19, 2023 |
| Last update date |
Jun 25, 2024 |
| Contact name |
Josh M Brickman |
| E-mail(s) |
joshua.brickman@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
reNEW
|
| Lab |
Brickman
|
| Street address |
3B Blegdamsvej
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL30172 |
| Series (2) |
| GSE232921 |
The primitive endoderm supports lineage plasticity to enable regulative development [RNA-seq] |
| GSE232926 |
The primitive endoderm supports lineage plasticity to enable regulative development |
|
| Relations |
| BioSample |
SAMN35217651 |
| SRA |
SRX20440436 |
