|
| Status |
Public on May 18, 2024 |
| Title |
AML-12 cells, overexpression, GM47544-2 |
| Sample type |
SRA |
| |
|
| Source name |
AML-12
|
| Organism |
Mus musculus |
| Characteristics |
cell line: AML-12
cell type: hepatocyte
genotype: GM47544 overexpressed
|
| Growth protocol |
AML-12 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol reagent.
A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw reads in fastq format were processed to remove contaminating adapter sequences using Trimmomatics
Quality distribution plot and base content distribution were generated by FASTQC
RNA-seq reads were aligned to the mm10 genome using STAR.
Read count extraction were performed using HTSeq.
Assembly: mm10
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
|
| |
|
| Submission date |
May 19, 2023 |
| Last update date |
May 18, 2024 |
| Contact name |
Qianqian Xiao |
| Organization name |
Tongji Hospital
|
| Department |
Departments of Internal Medicine
|
| Lab |
Division of Cardiology
|
| Street address |
1095# Jiefang Ave., Wuhan 430030 People’s Rep. of China
|
| City |
Wuhan |
| ZIP/Postal code |
430030 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE232866 |
Ubiquitination of hepatic APOC3 by GM47544 prevents hypertriglyceridemia and ameliorates atherosclerosis |
|
| Relations |
| BioSample |
SAMN35177437 |
| SRA |
SRX20435425 |
