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Sample GSM737553 Query DataSets for GSM737553
Status Public on Jun 07, 2011
Title dental follicle cells DLX3 1
Sample type RNA
 
Source name dental follicle cells in standard medium
Organism Homo sapiens
Characteristics cell type: primary dental progenitor stem cells
overexpression: DLX3
Treatment protocol Transfection was performed using FUGEN®HD (Roche) kit and the expression plasmid pCMV-V5DLX3 (pDLX3), which expresses DLX3 under the control of the constitutive CMV promoter and as control an empty vector without an insert.
Growth protocol Cell were grown in DMEM medium (PAA, Pasching, Austria) at 37°C in 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the kit NucleoSpin® RNA II kit (Macherey Nagel, Düren, Germany) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
Label biotin
Label protocol Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
 
Hybridization protocol Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.0 ST array was carried out overnight with 1biotin-labeled cRNA in a hybridization oven 640
Scan protocol GeneChip Scanner 3000 (Affymetrix)
Description Gene expression of dental follicle cells after 48 hours of transfection with a DLX3 plasmid
Data processing Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software
For the creation of the summarized log-signal the robust multi-chip analysis (RMA) algorithm was used
 
Submission date Jun 06, 2011
Last update date Jun 07, 2011
Contact name Christian Morsczeck
E-mail(s) christian.morsczeck@klinik.uni-regensburg.de
Organization name University of Regensburg
Street address Franz-Josef Strauss Allee 11
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platform ID GPL11532
Series (1)
GSE29753 Gene expression profiles of dental follicle cells after 48 hours of transfection in vitro with a DLX3 plasmid and an empty vector

Data table header descriptions
ID_REF
VALUE log RMA signal

Data table
ID_REF VALUE
7896736 4.59
7896738 2.70
7896740 2.44
7896742 7.27
7896744 3.91
7896746 7.67
7896748 5.27
7896750 3.31
7896752 9.68
7896754 4.42
7896756 5.26
7896759 5.39
7896761 4.20
7896779 5.90
7896798 6.33
7896817 6.73
7896822 8.33
7896859 2.89
7896861 2.89
7896863 3.97

Total number of rows: 28827

Table truncated, full table size 366 Kbytes.




Supplementary file Size Download File type/resource
GSM737553_Sample_1.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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