|
| Status |
Public on Jun 07, 2011 |
| Title |
mad386 |
| Sample type |
RNA |
| |
|
| Source name |
retinoblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary tumor
|
| Treatment protocol |
all samples are untreated tissues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
details and references in manuscript
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng of total RNA (Expression Analysis Technical Manual, 2005-2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
primary tumor
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.5 using the RMA algorithmn
|
| |
|
| Submission date |
Jun 01, 2011 |
| Last update date |
Jun 07, 2011 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE29683 |
Coexpression of Normally Incompatible Developmental Pathways in Retinoblastoma Genesis [human tumor/cell line data] |
| GSE29686 |
Coexpression of Normally Incompatible Developmental Pathways in Retinoblastoma Genesis |
|
