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Sample GSM73541 Query DataSets for GSM73541
Status Public on Sep 20, 2006
Title LOF_stage13_replicate1
Sample type RNA
 
Channel 1
Source name stage13 embryo mutant
Organism Drosophila melanogaster
Characteristics gcm mutant embryos automated sorted
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage13 embryo wt
Organism Drosophila melanogaster
Characteristics Oregon R wildtype embryos
Extracted molecule polyA RNA
Label Cy5
 
 
Description gcmN7-4 mutant flies were balanced with either Kr-GFP (for stages 10 to 13) or Ubi-GFP (for stages 14 to 16) balancer chromosomes. Eggs were collected as described above, transferred into PBS buffer supplemented with 0.3% Tween 20 and homozygous mutant embryos were automatically sorted with the CopasTM Select embryo sorter by their lack of GFP-expression. For wt control we used OregonR wildtype embryos that were treated the same way like the mutant embryos.Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide.
Data processing Genepix 5.0/MChips software
 
Submission date Sep 08, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R² (632/532)
F Pixels
B Pixels
Circularity
Sum of Medians (632/532)
Sum of Means (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R² (632/532) F Pixels B Pixels Circularity Sum of Medians (632/532) Sum of Means (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 VALUE
1 01_61_03_12 760 1780 100 3209 3241 1381 795 795 826 316 92 86 0 34603 32294 18540 485 485 557 371 100 100 0 0.071 0.077 0.079 0.082 1.820 0.072 0.878 80 360 100 36532 34255 2414 34118 2446 31809 -3.821
2 01_61_05_12 890 1770 100 913 892 326 772 772 820 315 28 3 0 549 669 498 435 435 532 426 17 10 0 1.237 0.513 1.386 1.519 5.702 0.222 0.115 80 283 100 255 354 141 114 120 234 0.307
3 01_61_07_12 1020 1770 100 740 812 353 796 796 831 305 18 5 0 324 352 189 291 291 311 201 25 2 0 -1.697 0.262 2.216 2.690 4.461 10.063 0.013 80 273 100 -23 77 -56 33 16 61 0
4 01_61_09_12 1160 1770 100 799 831 333 770 770 821 309 21 6 0 384 388 177 265 265 279 171 42 11 0 0.244 0.496 1.677 1.288 4.029 25.820 0.003 80 289 100 148 184 29 119 61 123 -2.037
5 01_61_11_12 1280 1780 60 1066 1112 423 756 756 823 368 46 15 0 1363 1355 190 329 329 374 190 100 100 0 0.300 0.347 0.413 0.372 2.354 0.718 0.072 32 115 100 1344 1382 310 1034 356 1026 -1.738
6 01_61_13_12 1410 1780 80 3110 3096 787 890 890 905 355 100 94 0 8242 7932 2161 409 409 423 191 100 100 0 0.283 0.293 0.288 0.284 1.355 0.291 0.820 52 220 100 10053 9729 2220 7833 2206 7523 -1.819
7 01_61_15_12 1550 1790 80 6768 6377 2267 860 860 889 336 100 100 0 21079 19134 7159 443 443 468 225 100 100 0 0.286 0.295 0.296 0.287 1.368 0.286 0.919 52 191 100 26544 24208 5908 20636 5517 18691 -1.804
8 01_61_01_24 1680 1780 90 8787 8091 2044 922 922 931 364 100 100 0 26538 22677 8682 447 447 589 563 100 100 0 0.301 0.322 0.316 0.350 1.413 0.295 0.915 52 271 100 33956 29399 7865 26091 7169 22230 -1.730
9 01_61_03_24 1840 1780 60 1116 1119 419 803 803 861 354 43 15 0 992 996 175 293 293 333 223 100 90 0 0.448 0.450 0.651 0.519 2.121 1.483 0.051 32 136 100 1012 1019 313 699 316 703 -1.159
10 01_61_05_24 1970 1770 100 865 912 412 806 806 828 320 31 8 0 411 428 161 241 241 260 163 53 16 0 0.347 0.567 1.457 1.548 5.097 17.081 0.009 80 325 100 229 293 59 170 106 187 -1.527
11 01_61_07_24 2100 1770 100 869 892 351 809 809 817 313 20 10 0 368 368 183 306 306 331 180 26 8 0 0.968 1.339 2.594 2.229 4.212 30.400 0.002 80 272 100 122 145 60 62 83 62 -0.047
12 01_61_09_24 2240 1770 100 825 863 346 826 826 845 356 16 3 0 609 604 222 368 368 376 188 61 25 0 -0.004 0.157 1.033 0.860 3.493 19.323 0.004 80 262 100 240 273 -1 241 37 236 0
13 01_61_11_24 2370 1770 100 829 892 322 827 827 831 322 20 3 0 467 475 194 382 382 394 187 28 8 0 0.024 0.699 1.562 1.649 3.694 22.718 0.002 80 262 100 87 158 2 85 65 93 -5.409
14 01_61_13_24 2510 1770 100 813 816 305 791 791 812 319 12 2 0 449 475 216 360 360 374 185 32 17 0 0.247 0.217 1.525 1.307 6.985 0.026 0.001 80 262 100 111 140 22 89 25 115 -2.016
15 01_61_15_24 2640 1770 100 737 783 307 785 785 802 320 16 2 0 381 412 233 347 347 356 166 33 12 0 -1.412 -0.031 1.463 1.550 4.368 23.823 0.002 80 262 100 -14 63 -48 34 -2 65 0
16 01_62_01_12 2780 1770 100 773 801 310 787 787 803 317 20 3 0 408 419 167 411 411 402 179 18 0 0 4.667 1.750 1.810 1.631 3.351 87.313 0.000 80 262 100 -17 22 -14 -3 14 8 2.222
17 01_62_03_12 2910 1770 100 824 861 384 838 838 860 370 17 5 0 415 409 181 414 414 404 172 20 0 0 -14.000 -4.600 1.499 1.636 4.464 61.772 0.001 80 276 100 -13 18 -14 1 23 -5 0
18 01_62_05_12 3050 1770 100 843 888 261 830 830 854 351 12 0 0 429 449 173 415 415 430 182 17 5 0 0.929 1.706 1.714 1.597 6.563 0.037 0.001 80 331 100 27 92 13 14 58 34 -0.107
19 01_62_07_12 3180 1770 100 746 814 316 835 835 851 301 13 5 0 463 450 163 508 508 505 179 6 0 0 1.978 0.362 1.719 1.812 5.070 1.518.137 0.000 80 302 100 -134 -79 -89 -45 -21 -58 0.984
20 01_62_09_12 3310 1770 100 842 847 321 826 826 854 319 16 1 0 417 436 136 487 487 482 182 3 1 0 -0.229 -0.412 2.037 2.008 4.200 96.175 0.000 80 276 100 -54 -30 16 -70 21 -51 0

Total number of rows: 47616

Table truncated, full table size 8892 Kbytes.




Supplementary data files not provided
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