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Sample GSM733739 Query DataSets for GSM733739
Status Public on Jun 02, 2011
Title Bernstein_Osteobl_H3K27ac
Sample type SRA
Source name Osteobl
Organism Homo sapiens
Characteristics lab: Broad
lab description: Bernstein - Broad Institute
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell organism: human
cell description: osteoblasts (NHOst)
cell lineage: mesoderm
cell sex: U
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: abcam
antibody vendorid: ab4729
treatment: None
treatment description: No special treatment or protocol applies
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH001056
replicate: 1,2
softwareversion: ScriptureVPaperR3
Biomaterial provider Lonza
Growth protocol Osteoblast_Bernstein_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol For extraction protocol details see:
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
Data processing For data processing details see:
Submission date May 27, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platform ID GPL9115
Series (1)
GSE29611 Histone Modifications by ChIP-seq from ENCODE/Broad Institute
SRA SRX067490
BioSample SAMN00622160
Named Annotation GSM733739_hg19_wgEncodeBroadHistoneOsteoblH3k27acStdSig.bigWig

Supplementary file Size Download File type/resource
GSM733739_hg19_wgEncodeBroadHistoneOsteoblH3k27acStdAlnRep1.bam 741.1 Mb (ftp)(http) BAM
GSM733739_hg19_wgEncodeBroadHistoneOsteoblH3k27acStdAlnRep2.bam 1.6 Gb (ftp)(http) BAM
GSM733739_hg19_wgEncodeBroadHistoneOsteoblH3k27acStdPk.broadPeak.gz 1.5 Mb (ftp)(http) BROADPEAK
GSM733739_hg19_wgEncodeBroadHistoneOsteoblH3k27acStdSig.bigWig 613.0 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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