|
| Status |
Public on May 09, 2023 |
| Title |
Donor AU80537 CAR + dSHP2 Stimulated |
| Sample type |
RNA |
| |
|
| Source name |
Transduced T cells
|
| Organism |
Homo sapiens |
| Characteristics |
donor: Donor AU80537
cell type: CAR T cells
condition: CAR + dSHP2, Stimulated, 24hr
|
| Treatment protocol |
T Cells were stimulated for 24 hours with Milteny Transact then a further 24 hours with IL7 and IL15 and transduced with retrovirus encoding a CD19 CAR or a CD19 CAR + the dSHP module. Four dats following transduction
|
| Growth protocol |
T Cells were maintained in RPMI medium supplemented with 10% fetal calf serum and 100U/ml IL2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a Qiagen RNAeasy mini kit plus DNAse treatment and frozen at -80C until analysis
|
| Label |
n/a
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
CodeSet Hybridization Setup MAN-10056 (https://www.nanostring.com/download_file/view/863/3778) + nCounter
|
| Scan protocol |
SPRINT® Profiler User Manual MAN-10017 (https://www.nanostring.com/download_file/view/254/3778)
|
| Description |
mRNA was extracted following transduction and the indicated treatment of the T cells and assessed for gene expression using Nanostring
|
| Data processing |
Matrix values values were generated by normalization of geometic means of gene expression to defined housekeeping genes utilizing NSolver 4.0 software
|
| |
|
| Submission date |
May 05, 2023 |
| Last update date |
May 10, 2023 |
| Contact name |
Simon Thomas |
| E-mail(s) |
s.thomas@autolus.com
|
| Organization name |
Autolus
|
| Street address |
The MediaWorks, 191 Wood Lane
|
| City |
London |
| ZIP/Postal code |
W12 7FP |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL32141 |
| Series (1) |
| GSE231743 |
RNA Seq comparing HD37 with dSHP2-HD37 |
|
