|
| Status |
Public on May 25, 2011 |
| Title |
Embryonic stem cell L139_ESC_17 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic stem cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Embryonic stem cell
source cell line: R1
well: A3
|
| Growth protocol |
ES R1 cells were cultured as previously described (Moliner et al. 2008). MEFs were grown in DMEM with 10% FBS, 1x penicillin/streptomycin, 1x Glutamax and 0.05 mM 2-mercaptoethanol. All reagents were from Invitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Custom protocol designed to capture the 5' end of transcripts, and maintain strand-information. See accompanying publication for details.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Barcodes were removed and reads were aligned using Bowtie.
|
| |
|
| Submission date |
May 23, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Sten Linnarsson |
| E-mail(s) |
sten.linnarsson@ki.se
|
| Phone |
+46852487577
|
| URL |
http://ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
MBB
|
| Lab |
Molecular Neurobiology
|
| Street address |
Scheeles väg 2
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL9250 |
| Series (1) |
| GSE29087 |
Characterization of the single-cell (ES and MEF) transcriptional landscape by highly multiplex RNA-Seq |
|
| Relations |
| SRA |
SRX064514 |
| BioSample |
SAMN00619250 |
