|
Status |
Public on May 25, 2011 |
Title |
Embryonic stem cell L139_ESC_3 |
Sample type |
SRA |
|
|
Source name |
Embryonic stem cell
|
Organism |
Mus musculus |
Characteristics |
cell type: Embryonic stem cell source cell line: R1 well: C1
|
Growth protocol |
ES R1 cells were cultured as previously described (Moliner et al. 2008). MEFs were grown in DMEM with 10% FBS, 1x penicillin/streptomycin, 1x Glutamax and 0.05 mM 2-mercaptoethanol. All reagents were from Invitrogen.
|
Extracted molecule |
total RNA |
Extraction protocol |
Custom protocol designed to capture the 5' end of transcripts, and maintain strand-information. See accompanying publication for details.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer II |
|
|
Data processing |
Barcodes were removed and reads were aligned using Bowtie.
|
|
|
Submission date |
May 23, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Sten Linnarsson |
E-mail(s) |
sten.linnarsson@ki.se
|
Phone |
+46852487577
|
URL |
http://ki.se
|
Organization name |
Karolinska Institutet
|
Department |
MBB
|
Lab |
Molecular Neurobiology
|
Street address |
Scheeles väg 2
|
City |
Stockholm |
ZIP/Postal code |
17177 |
Country |
Sweden |
|
|
Platform ID |
GPL9250 |
Series (1) |
GSE29087 |
Characterization of the single-cell (ES and MEF) transcriptional landscape by highly multiplex RNA-Seq |
|
Relations |
SRA |
SRX064500 |
BioSample |
SAMN00619236 |