|
| Status |
Public on May 29, 2024 |
| Title |
soy peptide-diet fed group-house mouse #2 [GP2] |
| Sample type |
SRA |
| |
|
| Source name |
Fecal
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Fecal
cell type: gut microbial
genotype: WT
treatment: soy peptide diet / group-house
|
| Treatment protocol |
Group-house and social isolation mice were fed either a control diet, containing 14% casein, or a soy peptide diet, containing 7% casein plus 7% soy peptides for 4 weeks post-weaning.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Mouse fecal samples were collected directly into tubes from which microbial DNA were extracted.
The V3-V4 regions of 16S rRNA genes were amplified using PCR with individually barcoded universal primers and sequenced by MiSeq next-generation sequencer with an average depth of 75,000 reads per sample.
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
library strategy: 16S rRNA-seq
Flora Genesis software
Microbial species were identified based on 16S rRNA gene sequence data using Flora Genesis software.
Assembly: BLAST 2.2.22 / gg_otus-13_8-release
Supplementary files format and content: Excel file includes microbial species-specific read number for each Sample
|
| |
|
| Submission date |
May 03, 2023 |
| Last update date |
May 29, 2024 |
| Contact name |
Hideki Tamura |
| E-mail(s) |
h-tamura@hoshi.ac.jp
|
| Phone |
+81-3-5498-5845
|
| Organization name |
Hoshi University
|
| Street address |
2-4-41 Ebara
|
| City |
Shinagawa-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
142-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE231557 |
Effects of chronic ingestion of soy peptide supplementation on gut microbial composition during juvenile social isolation |
|
| Relations |
| BioSample |
SAMN34566604 |
| SRA |
SRX20204054 |
