|
| Status |
Public on Feb 27, 2012 |
| Title |
LA+HYP_2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
growth conditions: media with 10 mM lactic acid (pH 6.7) in hypoxia (1% pO2)
|
| Treatment protocol |
MCF7 cells were exposed to control condition (ambient air ~21% O2, no lactate and neutral pH), lactic acidosis (ambient air, 10 mM Lactate and pH 6.7), hypoxia (1% pO2, no lactate and neutral pH) and the combined lactic acidosis and hypoxia (1% pO2, 10 mM Lactate and pH 6.7) condition for 24 hours.
|
| Growth protocol |
MCF7 breast cancer cells were cultured in DMEM (GIBCO-11995) supplemented with 10% fetal bovine serum and 1× antibiotics (penicillin, 10000UI/ml and streptomycin, 10000UI/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted by miRVana kits (Ambion) with standard protocol.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
gene expression data from MCF7 cells
|
| Data processing |
Expression Console (Affymetrix) was used to generate the RMA file. Cluster 3.0 was used for hierarhical clustering.
|
| |
|
| Submission date |
May 19, 2011 |
| Last update date |
Feb 27, 2012 |
| Contact name |
Xiaohu Tang |
| E-mail(s) |
xt2@duke.edu
|
| Organization name |
Duke University
|
| Department |
Department of Molecular Genetics and Microbiology
|
| Street address |
CIEMAS Rm 2133, 101 Science Dr.
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE29406 |
The genomic analysis of the interaction between the lactic acidosis and hypoxia response |
|
