|
| Status |
Public on Jun 07, 2013 |
| Title |
Control - #588 - hippocampus CA3 - Male - 85yr |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus - CA3
|
| Organism |
Homo sapiens |
| Characteristics |
subjectnumber: 588
regioninhippocampus: CA3
gender: Male
age(years): 85
postmorteminterval(hours): 5
plaquescore(0-3): 1
braakstage(0-6): 2
apoegenotype(alleles): 2,3
diseaseduration(years): NA
brainbank: OHSU
chipnumber(batch): 5298416058
locationonchip: I
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using a scalpel CA1 and CA3 subfields were dissected from sectioned but unlabeled tissue on dry ice and immediately placed into elution buffer for RNA extraction. Total RNA from each sample was isolated using the RNeasy Micro Kit with DNase I treatment (QIAGEN; Valencia, CA), then tested for quality on the Agilent 2100 Bioanalyzer using RNA 6000 Nano Chips (Agilent Technologies; Palo Alto, CA). This extraction followed the protocols provided by the manufacturers.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared by the Southern California Genotyping Consortium (www.semel.ucla.edu/core/scgc), using their standard procedure.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Control - #588 - hippocampus CA3 - Male - 85yr
|
| Data processing |
The data were normalised using quantile normalisation with normalize.quantiles() in R
|
| |
|
| Submission date |
May 18, 2011 |
| Last update date |
Jun 07, 2013 |
| Contact name |
Jeremy Miller |
| E-mail(s) |
jeremyinla@gmail.com
|
| Organization name |
Allen Institute for Brain Science
|
| Street address |
615 Westlake Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE29378 |
Genes and Pathways Underlying Regional and Cell Type Changes in Alzheimer's Disease |
|
