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Sample GSM714645 Query DataSets for GSM714645
Status Public on May 15, 2011
Title RNA_Ago2_PAR-CLIP_completeT1_repB
Sample type SRA
 
Source name HEK293
Organism Homo sapiens
Characteristics cell line: HEK293
clip variant: PAR-CLIP
enzymatic digestion: complete T1 digestion
protein: Ago2
Treatment protocol For samples subjected to PAR-CLIP, 4-Thiouridine was added to cells grown in monolayer at 1mM final concentration 12-14 hours prior to crosslinking at 365 nm UV. For samples subjected to CLIP, the cells were directly crosslinked at 254 nm UV.
Growth protocol HEK293 cells were routinely grown in DMEM supplemented with 10% FCS in the presence of antibiotics.
Extracted molecule cytoplasmic RNA
Extraction protocol CLIP, PAR-CLIP
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
 
Data processing The raw reads were processed on the CLIPZ server (Khorshid et al., Nucleic Acids Research 2011, 39:D245)
 
Submission date Apr 26, 2011
Last update date May 15, 2019
Contact name Jean Hausser
Organization name Weizmann Institute of Science
Street address Herzl St.
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platform ID GPL10999
Series (2)
GSE28859 A quantitative analysis of CLIP methods for identifying binding sites of RNA-binding proteins (CLIP)
GSE28865 A quantitative analysis of CLIP methods for identifying binding sites of RNA-binding proteins
Relations
SRA SRX058620
BioSample SAMN00260289

Supplementary file Size Download File type/resource
GSM714645_Ago2_PAR-CLIP_B.fa.gz 795.1 Kb (ftp)(http) FA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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