|
| Status |
Public on Jul 16, 2024 |
| Title |
Vero_Rep1_Unmodified |
| Sample type |
SRA |
| |
|
| Source name |
Vero Cells
|
| Organism |
Chlorocebus aethiops |
| Characteristics |
chemical modification: dimethyl sulfoxide
|
| Treatment protocol |
At 4dpi, each sample was collected and treated with either 200mM NAI (final) or equivalent volume DMSO
|
| Growth protocol |
Vero and C6/36 cells were cultured in 10cm tissue culture plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
cDNA was prepared from modified RNA using gene-specific RT primers, followed by generation of amplicons with gene-specific primers. Nextera-XT kit was used for library construction
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
West_Nile_virus_Unmod_R1
|
| Data processing |
ShapeMapper
Library strategy: SHAPE-MaP
|
| |
|
| Submission date |
Mar 28, 2023 |
| Last update date |
Jul 16, 2024 |
| Contact name |
Nicholas Huston |
| Organization name |
Yale University
|
| Street address |
260 Whitney Avenue, YSB 308
|
| City |
New Haven |
| State/province |
Connecticut |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL22096 |
| Series (1) |
| GSE228446 |
West Nile virus genome harbors essential riboregulatory elements with conserved and host-specific functional roles |
|
| Relations |
| BioSample |
SAMN33957395 |
| SRA |
SRX19801794 |
