|
| Status |
Public on May 29, 2023 |
| Title |
mouse ESC, CS5246 EZH2 |
| Sample type |
SRA |
| |
|
| Source name |
embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: CAST/B6 Male Rosa26-RMCE
cell type: embryonic stem cells
genotype: WT
antibody: CS5246 EZH2
|
| Growth protocol |
RMCE embryonic Stem Cells were maintained in DMEM supplemented with 15% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 2mM DSG and 1% formaldehyde
NEB End Repair, purified with AMPure XP Beads, A-Tailing with Klenow Fragment, Ligated 1uM TruSeq Adapters
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
aligned to mm10 b6 and cast genomes using bowtie2
filtered for reads that passed q30 quality filter
peaks were called using macs2 callPeak
used bedtools intersect to find peak overlaps
used subread featureCounts to assign sequencing reads to called peaks
Assembly: mm10
Supplementary files format and content: wig
Supplementary files format and content: bam file of aligned and filtered data
|
| |
|
| Submission date |
Mar 21, 2023 |
| Last update date |
May 29, 2023 |
| Contact name |
Joseph Mauro Calabrese |
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Pharmacology
|
| Lab |
Calabrese
|
| Street address |
120 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE227890 |
A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB [ChIP-seq] |
| GSE227893 |
A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB |
|
| Relations |
| BioSample |
SAMN33845681 |
| SRA |
SRX19742258 |
