|
| Status |
Public on Jan 17, 2024 |
| Title |
Arpp21 KO5 |
| Sample type |
SRA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
cell line: primary cells
cell type: CD4 T cells
genotype: Arpp21 KO
treatment: FACS cell sodting, naive T cells
|
| Treatment protocol |
FACS purified
|
| Growth protocol |
primary cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
stabdard mRNA extraction kit
Library preparation kit: Takara SMARTer mouse TCR alpha beta profiling kit
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
Takara SMARTer mouse TCR alpha beta profiling kit
|
| Data processing |
data processing and initial analysis were done by Genohub
Supplementary files format and content: .xlsx files contain detailed information about each identified TCR alpha and TCR beta clone. This includes clone number, frequency, CDR3 nucleotide sequence and information on the V-fagment used.
Library strategy: TCR-seq
|
| |
|
| Submission date |
Mar 01, 2023 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Marcin Lyszkiewicz |
| E-mail(s) |
marcin.lyszkiewicz@googlemail.com, marcin.lyszkiewicz@uni-ulm.de
|
| Organization name |
Ulm University Medical Center
|
| Department |
Dpt. of Pediatrics and Adolescent Medicine
|
| Street address |
Eythstr. 24
|
| City |
Ulm |
| ZIP/Postal code |
89073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE226369 |
Efect of Arpp21 deficiency on TCR repertoire diversity |
|
| Relations |
| BioSample |
SAMN33560431 |
| SRA |
SRX19544685 |
