|
| Status |
Public on Jan 10, 2012 |
| Title |
SJERG020 |
| Sample type |
RNA |
| |
|
| Source name |
Leukemic cells obtained from bone marrow or blood at diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: tumor cells obtained at diagnosis
subtype: 7_Other
|
| Growth protocol |
No growth protocol. Cells were obtained from bone marrow or blood from children with acute lymphoblastic leukemia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix One-Cycle protocol from 1 ug of total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
B-precursor ALL with miscellaneous karyotype
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Charles G Mullighan |
| E-mail(s) |
charles.mullighan@stjude.org
|
| Phone |
901-595-3387
|
| Fax |
901-595-5947
|
| URL |
http://www.stjude.org/mullighan
|
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Pathology
|
| Lab |
Mullighan
|
| Street address |
262 Danny Thomas Place, MS 342
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105-3678 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE33315 |
Discovery of novel recurrent mutations and rearrangements in early T-cell precursor acute lymphoblastic leukaemia by whole genome sequencing |
|
| Relations |
| Reanalyzed by |
GSE122505 |
